Study on chemical constituents from Mastic (Pistacia lentiscus) / 中国中药杂志

Phytochemical investigation on the methanolic extract of Mastic by using various chromatographic techniques led to the isolation of 9 compounds. Based on the analysis of spectroscopic data(NMR and MS) and/or comparisons with the data reported in the literature, their structures were elucidated as 3β,8α,13-trihydroxypolypoda-14-methoxy-14-methyl-17,21-diene(1), 4-hydroxymyrtenal(2),3-methyl-6-(prop-1-en-2-yl)cyclohex-3-ene-1, 2-diol(3), 2-oxo-Δ~3-4,5,5-trimethylcyclopentynyl acidic acid(4),(1S,2R,3R,5R)-6,6-dimethyl-4-methylidenebicyclo[3.1.1]-heptane-2,3-diol(5),(4R)-1-methyl-4-(1-hydroxyisopropyl)cyclohexene-6-one(6), 6,6-dimethyl-4-hydroxy[3.1.1]hept-2-ene-2-carboxylic acid(7), 6,6-dimethyl[3.1.1]hept-2-ene-2-carboxylic acid(8), 6,6-dimethyl-4-oxobicyclo[3.1.1]hept-2-ene-2-carboxylic acid(9). Compound 1 is a new compound and 2-9 were isolated from this species for the first time. In vitro cytotoxicity assay results indicated that compounds 1, 6 and 7 showed significant inhibitory effects against human lung cancer cell line A549 with IC_(50) values of 20.4, 25.1 and 22.5 μmoL·L~(-1).

Chemical constituents from fruiting bodies of Amauroderma rude / 中草药

Objective: To study the chemical constituents from the fruiting bodies in Amauroderma rude of family Ganodermataceae. Methods: The constituents were separated by column chromatography and their structures were elucidated by spectral data analyses. The cytotoxicities of compounds were evaluated using MTT methods. Results: Twelve compounds were isolated and identified from the fruiting bodies of A. rude and were identified as diptoindonesin D (1), 6-deoxyjacareubin (2), jacareubin (3), 1H-indole-3-carboxylic acid (4), methyl 3,4-dihydroxybenzoate (5), 3,4-dihydroxyphenylethanol (6), 3β-hydroxy-7, 22E-dien-ergosta (7), 3β, 7α-dihydroxy-8,22E-5α, 6α-epoxyergosta (8), 3β-hydroxy-7α-methoxy-8 (14), 22E-dien-5α, 6α-epoxyergosta (9), ergosterol 5α, 8α-peroxide (10), 3β-5β-8β-trihydroxy-6, 22E-ergosta (11), and 3β, 5α-6β-trihydroxy-7, 22E-dien-ergosta (12). Compounds 2 and 3 exhibited the cytotoxic activities against HL-60, SMMC-7721, A-549, MCF-7, and SW-480 cell lines and compound 9 showed the cytotoxic activities against HL-60, MCF-7, and SW-480 cell lines. Conclusion: All the compounds are obtained from this fungus for the first time. Compounds 2,3, and 9 show the definite cytotoxicities against five cell lines.